The reference image is the base image on which the alignment markers, digit ROIs and anlog ROIs will be defined.

Firstly the actual saved reference image is shown. If you start with the setup from scratch a default image is shown as placeholder. Use the button "Create New Reference" to start creation of your own reference image. After selecting the button a new image will be taken all configured parameter will be applied to the shown image. With the button "Update Image" you can update the image again (still all parameter get applied to the new image).

To have reliable evaluation processing a properly horizontal aligned evaluation area is mandatory. Using the parameters "Rotation angle" and "Rotation angle (Fine-tune)" the image can be rotated in both directions. The resulting rotation anlge is used to prerotate the image before the alignment algorithm is processed to compensate only small misalignments. Further information can be found in documenation: Reference Image

After setting up your reference image don't forget to save with the "Save New Reference" button!
NOTE: There is no need to perform a reboot after every saving or step. It's sufficient to reboot after all configuration steps (reference image, alignment, ROI configuration) are completed to activate new configuration.