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<li class="toctree-l1"><a class="reference internal" href="../ROI-Configuration/">ROIs (Regions of Interest)</a>
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<h1 id="alignment-references">Alignment References</h1>
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<p>The alignment references are used in every round to re-align the taken image to the reference coordinates.
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Two alignment structures must be defined and the taken image then in each round is shifted and rotated according to their position
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with the target to be in exactly the same position as the reference image.</p>
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<div class="admonition note">
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<p class="admonition-title">Note</p>
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<p>The alignment structures needs to be unique and have a good contrast.
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It is advised to have them as far apart as possible.</p>
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</div>
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<h2 id="precondition">Precondition</h2>
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<p>Please make sure to have setup your camera properly and taken a good <a href="../Reference-Image">Reference Image</a>.</p>
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<h2 id="define-two-reference-images">Define two Reference Images</h2>
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<p><img alt="" src="../img/initial_setup_2_alignment_marks.jpg" style="width:500px" /></p>
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<p>You can switch between this two marks with <code>(1)</code>.</p>
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<p>Then define the reference area in the image by either directly drag and drop with the mouse or use the input boxes below.
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To apply the currently marked image part you need to push <code>"Update Reference" (2)</code>. </p>
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<p>In some cases it might be useful to use a reference with a higher contrast. This can be achieved by pushing <code>Enhance Contrast" (3)</code>.
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The result will be calculated on the ESP32 - so be a bit patient, before you see it active.</p>
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<p>To save push <code>"Save to config.ini" (4)</code>.</p>
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<div class="admonition note">
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<p class="admonition-title">Note</p>
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<p>A reboot is not required at this point of time.</p>
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<p>As next you should define the <a href="../ROI-Configuration/">Digit and Analog ROIs</a>.</p>
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